Unbiased transcriptomics, 3D-cell culture and organs-on-a-chip. From applied science to basic science and back again

Speaker

Michael Miskelly, Department of Biomedical Science, Malmö University

Talk overview

This seminar will focus on three main themes. Initially there will be a discussion on how an unbiased transcriptomics approach following Roux-en-Y gastric bypass can highlight novel regulators of the incretin hormone GLP-1. Secondly, the benefits of single-cell RNA sequencing (scRNAseq) using SPLiT-seq will be highlighted alongside how scRNAseq can be utilised to identify dysregulated gene networks in patients with type 2 diabetes. The final theme will highlight how intestinal organoids and a gut-on-a-chip platform can be used to assess animal-microbiota evolution. The main methods I will discuss during this talk are bulk RNA sequencing, scRNAseq using SPLiT-seq, intestinal organoids and the Emulate organ-on-a-chip platform.

Fika will be provided. We look forward to seeing you there!

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